Items where Author is "Bustin, Stephen A."

Whale, Alexandra S., von der Heide, Eva K., Kohlenberg, Max, Brinckmann, Anja, Baedker, Silke, Karalay, Oezlem, Fernandez-Gonzalez, Ana, Busby, Eloise J., Bustin, Stephen A., Hauser, Heiko, Missel, Andreas, O'Sullivan, Denise M., Huggett, Jim F., Pfaffl, Michael W. and Nolan, Tania (2022) Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics. Methods, 201. pp. 5-14. ISSN 1095-9130

Bustin, Stephen A., Shipley, Gregory L., Kirvell, Sara, Mueller, Reinhold and Nolan, Tania (2022) RT-qPCR Detection of SARS-CoV-2: No Need for a Dedicated Reverse Transcription Step. International Journal of Molecular Sciences, 23 (3). p. 1303. ISSN 1422-0067

Bivins, Aaron, Kaya, Devrim, Bibby, Kyle, Simpson, Stuart L., Bustin, Stephen A., Shanks, Orin C. and Ahmed, Warish (2021) Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance. Water Research, 203. p. 117516. ISSN 1879-2448

Bustin, Stephen A., Kirvell, Sara, Huggett, Jim F. and Nolan, Tania (2021) RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm. International Journal of Molecular Sciences, 22 (16). p. 8702. ISSN 1422-0067

Bustin, Stephen A., Mueller, Reinhold D., Shipley, Gregory L. and Nolan, Tania (2021) COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies. International Journal of Molecular Sciences, 22 (5). p. 2459. ISSN 1422-0067

Huggett, Jim F., Benes, Vladimir, Bustin, Stephen A. ORCID: https://orcid.org/0000-0003-1870-6098, Garson, Jeremy A., Harris, Kathryn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D., Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W., Salit, Marc, Shipley, Gregory L., Vallone, Peter M., Vandesompele, Jo, Wittwer, Carl T. and Zeichhardt, Heinz (2020) Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Clinical Chemistry, 66 (11). pp. 1369-1372. ISSN 1530-8561

Ilg, Marcus M., Stafford, Simon J., Mateus, Marta, Bustin, Stephen A., Carpenter, Michael J., Muneer, Asif, Bivalacqua, Trinity J., Ralph, David J. and Cellek, Selim (2020) Phosphodiesterase Type 5 Inhibitors and Selective Estrogen Receptor Modulators Can Prevent But Not Reverse Myofibroblast Transformation in Peyronie's Disease. Journal of Sexual Medicine, 17 (10). pp. 1848-1864. ISSN 1743-6109

Huggett, Jim F., Whale, Alexandra S., De Spiegelaere, Ward, Trypsteen, Wim, Nour, Afif Abdel, Bae, Young-Kyung, Benes, Vladimir, Burke, Daniel, Cleveland, Megan, Corbisier, Philippe, Devonshire, Alison S., Dong, Lianhua, Drandi, Daniela, Foy, Carole A., Garson, Jeremy A., He, Hua-Jun, Hellemans, Jan, Kubista, Mikael, Lievens, Antoon, Makrigiorgos, Mike G., Milavec, Mojca, Mueller, Reinhold D., Nolan, Tania, O’Sullivan, Denise M., Pfaffl, Michael W., Rödiger, Stefan, Romsos, Erica L., Shipley, Gregory L., Taly, Valerie, Untergasser, Andreas, Wittwer, Carl T., Bustin, Stephen A. and Vandesompele, Jo (2020) The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. Clinical Chemistry, 66 (8). pp. 1012-1029. ISSN 1530-8561

Bustin, Stephen A. and Nolan, Tania (2020) RT-qPCR Testing of SARS-CoV-2: A Primer. International Journal of Molecular Sciences. ISSN 1422-0067

Prattes, Juergen, Hoenigl, Martin, Zinke, Stefanie E.-M., Heldt, Sven, Eigl, Susanne, Johnson, Gemma L., Bustin, Stephen A., Stelzl, Evelyn and Kessler, Harald H. (2018) Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study. Mycoses, 61 (6). pp. 355-359. ISSN 1439-0507

Sanders, Rebecca, Bustin, Stephen A., Huggett, Jim and Mason, Deborah (2018) Improving the standardization of mRNA measurement by RT-qPCR. Biomolecular Detection and Quantification, 15. pp. 13-17. ISSN 2214-7535

Bustin, Stephen A. and Huggett, Jim (2017) qPCR primer design revisited. Biomolecular Detection and Quantification, 14. pp. 19-28. ISSN 2214-7535

Bustin, Stephen A. and Nolan, Tania (2017) Talking the talk, but not walking the walk: RT-qPCR as a paradigm for the lack of reproducibility in molecular research. European Journal of Clinical Investigation, 47 (10). pp. 756-774. ISSN 1365-2362

Bustin, Stephen A. and Wittwer, Carl T. (2017) MIQE: A Step Toward More Robust and Reproducible Quantitative PCR. Clinical Chemistry, 63 (9). pp. 1537-1538. ISSN 1530-8561

Bustin, Stephen A. (2017) How to speed up the polymerase chain reaction. Biomolecular Detection and Quantification, 12. pp. 10-14. ISSN 2214-7535

Bustin, Stephen A. (2017) The continuing problem of poor transparency of reporting and use of inappropriate methods for RT-qPCR. Biomolecular Detection and Quantification, 12. pp. 7-9. ISSN 2214-7535

Bustin, Stephen A. and Huggett, Jim F. (2017) Reproducibility of biomedical research – The importance of editorial vigilance. Biomolecular Detection and Quantification, 11. pp. 1-3. ISSN 2214-7535

Dhillon, Harvinder S., Johnson, Gemma L., Shannon, Mark, Greenwood, Christina, Roberts, Doug and Bustin, Stephen A. (2016) Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B. Biomolecular Detection and Quantification, 10. pp. 2-8. ISSN 2214-7535

Nolan, Tania and Bustin, Stephen A. (2016) PCR Technology: Current Innovations, Third Edition. CRC Press, Boca Raton, FL. ISBN 9781138198586

Ansari, Wahedul K., Parvej, Md Shafiullah, El Zowalaty, Mohamed E., Jackson, Sally, Bustin, Stephen A., Ibrahim, Adel K., El Zowalaty, Ahmed E., Rahman, Md Tanvir, Zhang, Han, Khan, Mohammad F. R., Ahamed, Md Mostakin, Rahman, Md Fasiur, Rahman, Marzia, Nazir, K. H. M. Nazmul Hussain, Ahmed, Sultan, Hossen, Md Liakot, Kafi, Md Abdul, Yamage, Mat, Debnath, Nitish C., Ahmed, Graba, Ashour, Hossam M., Masudur Rahman, Md, Noreddin, Ayman and Rahman, Md Bahanur (2016) Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh. Veterinary Microbiology, 193. pp. 49-59. ISSN 1873-2542

Bustin, Stephen A. (2016) Science in the UK - whereto now? Biomolecular Detection and Quantification, 9. A1-A4. ISSN 2214-7535

Sai-Giridhar, Priya, Al-Ramadhani, Salma, George, Dilushana, Gopinath, Preethi, Andrews, William, Jader, Samar, Brown, Shelley, Findlay, Alison, Arkoumani, Evdokia, Al-Sam, Salam, McKenzie, Jolanta G, Bradpiece, Howard, Jenkins, Stephanie, Aylwin, Anthony, Holt, Simon, Sharaiha, Yousef, Yiangou, Constantinos, Agrawal, Avi, McDowell, Amanda, Gabriel, Francis G, Jeffrey, Margaret, Agrawal, Neerja, Cree, Ian A, Mansel, Robert E, Keshtgar, Mo, McDermott, Nuala, El Sheikh, Soha, Wellsted, David, Collard, Jade, Chaplin, Hema, Landt, Olfert, Bustin, Stephen A., Sundaresan, Maryse and Sundaresan, Vasi (2016) A multicentre validation of Metasin: a molecular assay for the intraoperative assessment of sentinel lymph nodes from breast cancer patients. Histopathology, 68 (6). pp. 875-887. ISSN 1365-2559

Bustin, Stephen A. (2015) Book review: Molecular Biology of the Cell, Sixth Edition; ISBN: 9780815344643; and Molecular Biology of the Cell, Sixth Edition, The Problems Book; ISBN 9780815344537. International Journal of Molecular Sciences, 16 (12). p. 28123. ISSN 1422-0067

Elliott, Timothy R., Rayment, Neil B., Hudspith, Barry N., Hands, Rebecca E., Taylor, Kirstin, Parkes, Gareth C., Prescott, Natalie J., Petrovska, Liljana, Hermon-Taylor, John, Brostoff, Jonathan, Boussioutas, Alex, Mathew, Christopher G., Bustin, Stephen A. and Sanderson, Jeremy D. (2015) Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease. BMC Gastroenterology, 15. p. 75. ISSN 1471-230X

Ruijter, Jan M., Lefever, Steve, Anckaert, Jasper, Hellemans, Jan, Pfaffl, Michael W., Benes, Vladimir, Bustin, Stephen A., Vandesompele, Jo and Untergasser, Andreas (2015) RDML-Ninja and RDMLdb for standardized exchange of qPCR data. BMC Bioinformatics, 16 (1). p. 197. ISSN 1471-2105

Johnson, Gemma L., Sarker, Shah-Jalal, Nannini, Francesco, Ferrini, Arianna, Taylor, Emma, Lass-Flörl, Cornelia, Mutschlechner, Wolfgang, Bustin, Stephen A. and Agrawal, Samir G. (2015) Aspergillus-Specific Lateral-Flow Device and Real-Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis. Journal of Clinical Microbiology, 53 (7). pp. 2103-2108. ISSN 1098-660X

Greenwood, Christina, Ruff, David, Kirvell, Sara, Johnson, Gemma L., Dhillon, Harvinder S. and Bustin, Stephen A. (2015) Proximity assays for sensitive quantification of proteins. Biomolecular Detection and Quantification, 4. pp. 10-16. ISSN 2214-7535

Greenwood, Christina, Johnson, Gemma L., Dhillon, Harvinder S. and Bustin, Stephen A. (2015) Recent progress in developing proximity ligation assays for pathogen detection. Expert Review of Molecular Diagnostics, 15 (7). pp. 861-867. ISSN 1744-8352

Huggett, Jim F., O’Grady, Justin and Bustin, Stephen A. (2015) qPCR, dPCR, NGS – A journey. Biomolecular Detection and Quantification, 3. A1-A5. ISSN 2214-7535

Bustin, Stephen A. (2015) The reproducibility of biomedical research: sleepers awake! Biomolecular Detection and Quantification, 2. pp. 35-42. ISSN 2214-7535

Bustin, Stephen A., Dhillon, Harvinder S., Kirvell, Sara, Greenwood, Christina, Parker, Michael, Shipley, Gregory L. and Nolan, Tania (2015) Variability of the reverse transcription step: practical implications. Clinical Chemistry, 61 (1). pp. 202-212. ISSN 1530-8561

Vrtačnik, Peter, Kos, Spela, Bustin, Stephen A., Marc, Janja and Ostanek, Barbara (2014) Influence of trypsinization and alternative procedures for cell preparation before RNA extraction on RNA integrity. Analytical Biochemistry, 463. pp. 38-44. ISSN 1096-0309

Johnson, Gemma L., Nour, Afif A., Nolan, Tania, Huggett, Jim F. and Bustin, Stephen A. (2014) Minimum Information Necessary for Quantitative Real-Time PCR Experiments. In: Quantitative Real-Time PCR: Methods and Protocols. Methods in Molecular Biology, 1160 . Humana Press, New York, NY, pp. 5-17. ISBN 978-1-4939-0733-5

Bustin, Stephen A. and Johnson, Gemma L. (2014) Rapid evolution of technologies holds out much promise. Asian Hospital and Healthcare Management, 30.

Abdel Nour, Afif M., Azhar, Esam, Damanhouri, Ghazi and Bustin, Stephen A. (2014) Five years MIQE guidelines: The case of the Arabian countries. PLOS ONE, 9 (2). e88266. ISSN 1932-6203

Bustin, Stephen A., Nicholls, Ian A. and Iba, Michael (2014) International Journal of Molecular Science Best Paper Award 2014. International Journal of Molecular Sciences, 15 (1). pp. 1683-1685. ISSN 1422-0067

Dijkstra, Jeroen R., Van Kempen, Léon C., Nagtegaal, Iris D. and Bustin, Stephen A. (2014) Critical appraisal of quantitative PCR results in colorectal cancer research: Can we rely on published qPCR results? Molecular Oncology, 8 (4). pp. 813-818. ISSN 1878-0261

Bustin, Stephen A., Benes, Vladimir, Garson, Jeremy A., Hellemans, Jan, Huggett, Jim F., Kubista, Mikael, Mueller, Reinhold D., Nolan, Tania, Pfaffl, Michael W., Shipley, Gregory, Wittwer, Carl T., Schjerling, Peter, Day, Philip J., Abreu, Mónica, Aguado, Begoña, Beaulieu, Jean-François, Beckers, Anneleen, Bogaert, Sara, Browne, John A., Carrasco-Ramiro, Fernando, Ceelen, Liesbeth, Ciborowski, Kate, Cornillie, Pieter, Coulon, Stephanie, Cuypers, Ann, De Brouwer, Sara, De Ceuninck, Leentje, De Craene, Jurgen, De Naeyer, Hélène, De Spiegelaere, Ward, Deckers, Kato, Dheedene, Annelies, Durinck, Kaat, Ferreira-Teixeira, Margarida, Fieuw, Annelies, Gallup, Jack M., Gonzalo-Flores, Sandra, Goossens, Karen, Heindryckx, Femke, Herring, Elizabeth, Hoenicka, Hans, Icardi, Laura, Jaggi, Rolf, Javad, Farzad, Karampelias, Michael, Kibenge, Frederick, Kibenge, Molly, Kumps, Candy, Lambertz, Irina, Lammens, Tim, Markey, Amelia, Messiaen, Peter, Mets, Evelien, Morais, Sofia, Mudarra-Rubio, Alberto, Nakiwala, Justine, Nelis, Hilde, Olsvik, Pal A., Pérez-Novo, Claudina, Plusquin, Michelle, Remans, Tony, Rihani, Ali, Rodrigues-Santos, Paulo, Rondou, Pieter, Sanders, Rebecca, Schmidt-Bleek, Katharina, Skovgaard, Kerstin, Smeets, Karen, Tabera, Laura, Toegel, Stefan, Van Acker, Tim, Van den Broeck, Wim, Van der Meulen, Joni, Van Gele, Mireille, Van Peer, Gert, Van Poucke, Mario, Van Roy, Nadine, Vergult, Sarah, Wauman, Joris, Tshuikina-Wiklander, Marina, Willems, Erik, Zaccara, Sara, Zeka, Fjoralba and Vandesompele, Jo (2013) The need for transparency and good practices in the qPCR literature. Nature Methods, 10 (11). pp. 1063-1067. ISSN 1548-7105

El Zowalaty, Mohamed E., Bustin, Stephen A., Husseiny, Mohamed I. and Ashour, Hossam M. (2013) Avian influenza: virology, diagnosis and surveillance. Future Microbiology, 8 (9). pp. 1209-1227. ISSN 1746-0921

Bustin, Stephen A. (2013) Transparency of reporting in molecular diagnostics. International Journal of Molecular Sciences, 14 (8). pp. 15878-15884. ISSN 1422-0067

Bustin, Stephen A. and Nolan, Tania (2013) Analysis of mRNA expression by real-time PCR. In: Real-Time PCR: Advanced Technologies and Applications. Caister Academic Press, Poole, UK. ISBN 978-1-908230-22-5

Huggett, Jim F., Nolan, Tania and Bustin, Stephen A. (2013) MIQE: guidelines for the design and publication of a reliable real-time PCR assay. In: Real-Time PCR: Advanced Technologies and Applications. Caister Academic Press, Poole, UK. ISBN 978-1-908230-22-5

Al-Ramadhani, Salma, Sai-Giridhar, Priya, George, Dilushana, Gopinath, Preethi, Arkoumani, Evdokia, Jader, Samar, Sundaresan, Maryse, Salgado, Roberto, Larsimont, Dennis, Bustin, Stephen A. and Sundaresan, Vasi (2013) Metasin — An Intra-Operative RT-qPCR Assay to Detect Metastatic Breast Cancer in Sentinel Lymph Nodes. International Journal of Molecular Sciences, 14 (7). pp. 12931-12952. ISSN 1422-0067

Johnson, Gemma L., Sarker, Shah-Jalal, Hill, Kate, Tsitsikas, Dimitris A., Morin, Amelie, Bustin, Stephen A. and Agrawal, Samir G. (2013) Significant decline in galactomannan signal during storage of clinical serum samples. International Journal of Molecular Sciences, 14 (7). pp. 12970-12977. ISSN 1422-0067

Huggett, Jim F., Foy, Carole A., Benes, Vladimir, Emslie, Kerry, Garson, Jeremy A., Haynes, Ross, Hellemans, Jan, Kubista, Mikael, Mueller, Reinhold D., Nolan, Tania, Pfaffl, Michael W., Shipley, Gregory L., Vandesompele, Jo, Wittwer, Carl T. and Bustin, Stephen A. (2013) The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chemistry, 59 (6). pp. 892-902. ISSN 1530-8561

Bustin, Stephen A. (2013) Why there is no link between measles virus and autism. In: Recent Advances in Autism Spectrum Disorders: Volume I. InTech Open, Rijeka, Croatia, pp. 81-98. ISBN 978-953-51-1021-7

Bustin, Stephen A. and Murphy, Jamie (2013) RNA biomarkers in colorectal cancer. Methods, 59 (1). pp. 116-125. ISSN 1095-9130

Johnson, Gemma L., Nolan, Tania and Bustin, Stephen A. (2013) Real-time quantitative PCR, pathogen detection and MIQE. In: PCR Detection of Microbial Pathogens: Second Edition. Methods in Molecular Biology, 943 . Humana Press, New York, NY, pp. 1-16. ISBN 9781603273534