Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer

Sushentsev, Nikita, McLean, Mary A., Warren, Anne Y., Benjamin, Arnold J. V., Brodie, Cara, Frary, Amy, Gill, Andrew B., Jones, Julia, Kaggie, Joshua D., Lamb, Benjamin W., Locke, Matthew J., Miller, Jodi L., Mills, Ian G., Priest, Andrew N., Robb, Fraser J. L., Shah, Nimish, Schulte, Rolf S., Graves, Martin J., Gnanapragasam, Vincent J., Brindle, Kevin M., Barrett, Tristan and Gallagher, Ferdia A. (2022) Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer. Nature Communications, 13 (1). p. 466. ISSN 2041-1723

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Abstract

Hyperpolarised magnetic resonance imaging (HP 13C-MRI) is an emerging clinical technique to detect [1-13C]lactate production in prostate cancer (PCa) following intravenous injection of hyperpolarised [1-13C]pyruvate. Here we differentiate clinically significant PCa from indolent disease in a low/intermediate-risk population by correlating [1-13C]lactate labelling on MRI with the percentage of Gleason pattern 4 (%GP4) disease. Using immunohistochemistry and spatial transcriptomics, we show that HP 13C-MRI predominantly measures metabolism in the epithelial compartment of the tumour, rather than the stroma. MRI-derived tumour [1-13C]lactate labelling correlated with epithelial mRNA expression of the enzyme lactate dehydrogenase (LDHA and LDHB combined), and the ratio of lactate transporter expression between the epithelial and stromal compartments (epithelium-to-stroma MCT4). We observe similar changes in MCT4, LDHA, and LDHB between tumours with primary Gleason patterns 3 and 4 in an independent TCGA cohort. Therefore, HP 13C-MRI can metabolically phenotype clinically significant disease based on underlying metabolic differences in the epithelial and stromal tumour compartments.

Item Type:	Journal Article
Keywords:	Cancer imaging, Cancer metabolism, Prostate, Prostate cancer
Faculty:	Faculty of Health, Education, Medicine & Social Care
Depositing User:	Lisa Blanshard
Date Deposited:	09 Feb 2022 15:41
Last Modified:	21 Mar 2022 11:54
URI:	https://arro.anglia.ac.uk/id/eprint/707316

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