EphA2 Affects Development of the Eye Lens Nucleus and the Gradient of Refractive Index

Cheng, Catherine, Wang, Kehao, Hoshino, Masato, Uesugi, Kentaro, Yagi, Naoto and Pierscionek, Barbara K. (2022) EphA2 Affects Development of the Eye Lens Nucleus and the Gradient of Refractive Index. Investigative Ophthalmology & Visual Science, 63 (1). p. 2. ISSN 1552-5783

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Official URL: https://doi.org/10.1167/iovs.63.1.2


Purpose: Our studies in mouse eye lenses demonstrate that ephrin-A5 and EphA2 are needed for normal epithelial cells and lens transparency. We sought to determine whether EphA2 and ephrin-A5 are important for lens morphometrics, nucleus formation, and refractive index. Methods: We performed tissue morphometric measurements, electron microscopy, Western blots, and interferometric measurements using an X-ray synchrotron beam source to measure the gradient of refractive index (GRIN) to compare mouse lenses with genetic disruption of EphA2 or ephrin-A5. Results: Morphometric analysis revealed that although there is no change in the overall lens volume, there is a change in lens shape in both EphA2−/− lenses and ephrin-A5−/− lenses. Surprisingly, EphA2−/− lenses had small and soft lens nuclei different from hard lens nuclei of control lenses. SEM images revealed changes in cell morphology of EphA2−/− fiber cells close to the center of the lens. Inner EphA2−/− lens fibers had more pronounced tongue-and-groove interdigitations and formed globular membrane morphology only in the deepest layers of the lens nucleus. We did not observe nuclear defects in ephrin-A5−/− lenses. There was an overall decrease in magnitude of refractive index across EphA2−/− lenses, which is most pronounced in the nucleus. Conclusions: This work reveals that Eph-ephrin signaling plays a role in fiber cell maturation, nuclear compaction, and lens shape. Loss of EphA2 disrupts the nuclear compaction resulting in a small lens nucleus. Our data suggest that Eph-ephrin signaling may be required for fiber cell membrane reorganization and compaction and for establishing a normal GRIN.

Item Type: Journal Article
Keywords: GRIN, interdigitation, fiber cells, SEM, ephrin-A5
Faculty: Faculty of Health, Education, Medicine & Social Care
Depositing User: Ian Walker
Date Deposited: 07 Jan 2022 14:00
Last Modified: 16 Mar 2022 16:21
URI: https://arro.anglia.ac.uk/id/eprint/707240

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