COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies

Bustin, Stephen A. and Mueller, Reinhold D. and Shipley, Gregory L. and Nolan, Tania (2021) COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies. International Journal of Molecular Sciences, 22 (5). p. 2459. ISSN 1422-0067

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Official URL: http://dx.doi.org/10.3390/ijms22052459

Abstract

Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use.

Item Type: Journal Article
Keywords: COVID-19, SARS-CoV-2, molecular diagnostics, quantification cycle, RT-qPCR
Faculty: Faculty of Science & Engineering
COVID-19 Research Collection
SWORD Depositor: Symplectic User
Depositing User: Symplectic User
Date Deposited: 04 Mar 2021 15:17
Last Modified: 09 Sep 2021 18:51
URI: https://arro.anglia.ac.uk/id/eprint/706392

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