Abdelkader, Hamdy and Longman, Michael R. and Alany, Raid G. and Pierscionek, Barbara K. (2016) Phytosome-hyaluronic acid systems for ocular delivery of L-carnosine. International Journal of Nanomedicine, 11. p. 2815. ISSN 1178-2013
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Abstract
This study reports on L-carnosine phytosomes as an alternative for the prodrug N-acetyl-L-carnosine as a novel delivery system to the lens. L-carnosine was loaded into lipid-based phytosomes and hyaluronic acid (HA)-dispersed phytosomes. L-carnosine-phospholipid complexes (PC) of different molar ratios, 1:1 and 1:2, were prepared by the solvent evaporation method. These complexes were characterized with thermal and spectral analyses. PC were dispersed in either phosphate buffered saline pH 7.4 or HA (0.1% w/v) in phosphate buffered saline to form phytosomes PC1:1, PC1:2, and PC1:2 HA, respectively. These phytosomal formulations were studied for size, zeta potential, morphology, contact angle, spreading coefficient, viscosity, ex vivo transcorneal permeation, and cytotoxicity using primary human corneal cells. L-carnosine-phospholipid formed a complex at a 1:2 molar ratio and phytosomes were in the size range of 380–450 nm, polydispersity index of 0.12–0.2. The viscosity of PC1:2 HA increased by 2.4 to 5-fold compared with HA solution and PC 1:2, respectively; significantly lower surface tension, contact angle, and greater spreading ability for phytosomes were also recorded. Ex vivo transcorneal permeation parameters showed significantly controlled corneal permeation of L-carnosine with the novel carrier systems without any significant impact on primary human corneal cell viability. Ex vivo porcine lenses incubated in high sugar media without and with L-carnosine showed concentration-dependent marked inhibition of lens brunescence indicative of the potential for delaying changes that underlie cataractogenesis that may be linked to diabetic processes.
| Item Type: | Journal Article |
|---|---|
| Keywords: | lipoid S 75, phytosomes, L-carnosine, cataract, hyaluronate sodium, ocular delivery |
| Faculty: | ARCHIVED Faculty of Health, Social Care & Education (until September 2018) |
| Depositing User: | Lisa Blanshard |
| Date Deposited: | 19 Feb 2021 14:50 |
| Last Modified: | 09 Sep 2021 19:00 |
| URI: | https://arro.anglia.ac.uk/id/eprint/706332 |
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