Analytical and physicochemical characterisation of the senile cataract drug dipeptide β-alanyl-L-histidine (carnosine)

Abdelkader, Hamdy and Swinden, Julian and Pierscionek, Barbara K. and Alany, Raid G. (2015) Analytical and physicochemical characterisation of the senile cataract drug dipeptide β-alanyl-L-histidine (carnosine). Journal of Pharmaceutical and Biomedical Analysis, 114. pp. 241-246. ISSN 0731-7085

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Official URL: https://doi.org/10.1016/j.jpba.2015.05.025

Abstract

This study presents a simple but sensitive HPLC chromatographic method with a stability-indicating assay for determination and physicochemical characterisation of L-carnosine, a promising senile cataract prophylactic agent. Chromatographic analysis was conducted using a reverse phase (RP)-HPLC system and an isocratic mobile phase of 98% v/v trifluoroacetic acid (0.1% v/v) and 2% v/v acetonitrile with detection at 220 nm. L-carnosine was subjected to stress conditions to force its degradation using chemical and thermal agents and was subsequently detected from its degradation products using ESI–MS. The lipophilicity of the drug and 1:1 drug to phospholipid complex (PC) mol/mol was determined by estimating the partition coefficient (P). Lipophilicity was greatly enhanced when L-carnosine was formulated as a phospholipid complex using the solvent evaporation method. L-carnosine-phospholipid complex could be a promising approach for effective delivery to the human lens as offers a potential novel treatment for senile cataract.

Item Type: Journal Article
Keywords: L-carnosine, Senile cataract, Histidine, Dipeptide, Ocular delivery
Faculty: ARCHIVED Faculty of Health, Social Care & Education (until September 2018)
Depositing User: Lisa Blanshard
Date Deposited: 18 Feb 2021 15:42
Last Modified: 22 Feb 2021 14:55
URI: https://arro.anglia.ac.uk/id/eprint/706296

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