Cheng, Catherine, Parreno, Justin, Nowak, Roberta B., Biswas, Sondip K., Wang, Kehao, Hoshino, Masato, Uesugi, Kentaro, Yagi, Naoto, Moncaster, Juliet A., Lo, Woo-Kuen, Pierscionek, Barbara K. and Fowler, Velia M. (2019) Age-related changes in eye lens biomechanics, morphology, refractive index and transparency. Aging, 11 (24). pp. 12497-12531. ISSN 1945-4589
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Abstract
Life-long eye lens function requires an appropriate gradient refractive index, biomechanical integrity and transparency. We conducted an extensive study of wild-type mouse lenses 1-30 months of age to define common age-related changes. Biomechanical testing and morphometrics revealed an increase in lens volume and stiffness with age. Lens capsule thickness and peripheral fiber cell widths increased between 2 to 4 months of age but not further, and thus, cannot account for significant age-dependent increases in lens stiffness after 4 months. In lenses from mice older than 12 months, we routinely observed cataracts due to changes in cell structure, with anterior cataracts due to incomplete suture closure and a cortical ring cataract corresponding to a zone of compaction in cortical lens fiber cells. Refractive index measurements showed a rapid growth in peak refractive index between 1 to 6 months of age, and the area of highest refractive index is correlated with increases in lens nucleus size with age. These data provide a comprehensive overview of age-related changes in murine lenses, including lens size, stiffness, nuclear fraction, refractive index, transparency, capsule thickness and cell structure. Our results suggest similarities between murine and primate lenses and provide a baseline for future lens aging studies
Item Type: | Journal Article |
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Keywords: | fiber cell, strain, epithelial cell, cataract, stiffness |
Faculty: | Faculty of Health, Education, Medicine & Social Care |
Depositing User: | Ian Walker |
Date Deposited: | 15 Feb 2021 22:07 |
Last Modified: | 09 Sep 2021 18:54 |
URI: | https://arro.anglia.ac.uk/id/eprint/706277 |
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