Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Huggett, Jim F. and Benes, Vladimir and Bustin, Stephen A. and Garson, Jeremy A. and Harris, Kathryn and Kammel, Martin and Kubista, Mikael and McHugh, Timothy D. and Moran-Gilad, Jacob and Nolan, Tania and Pfaffl, Michael W. and Salit, Marc and Shipley, Gregory L. and Vallone, Peter M. and Vandesompele, Jo and Wittwer, Carl T. and Zeichhardt, Heinz (2020) Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2. Clinical Chemistry, 66 (11). pp. 1369-1372. ISSN 1530-8561

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Official URL: https://doi.org/10.1093/clinchem/hvaa214

Abstract

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.

Item Type: Journal Article
Keywords: Contamination, SARS-CoV-2, Molecular Diagnosis
Faculty: COVID-19 Research Collection
Faculty of Health, Education, Medicine & Social Care
Depositing User: Unnamed user with email stephen.bustin@anglia.ac.uk
Date Deposited: 22 Sep 2020 10:47
Last Modified: 21 Jan 2021 16:53
URI: https://arro.anglia.ac.uk/id/eprint/705874

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