The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Huggett, Jim F. and Whale, Alexandra S. and De Spiegelaere, Ward and Trypsteen, Wim and Nour, Afif Abdel and Bae, Young-Kyung and Benes, Vladimir and Burke, Daniel and Cleveland, Megan and Corbisier, Philippe and Devonshire, Alison S. and Dong, Lianhua and Drandi, Daniela and Foy, Carole A. and Garson, Jeremy A. and He, Hua-Jun and Hellemans, Jan and Kubista, Mikael and Lievens, Antoon and Makrigiorgos, Mike G. and Milavec, Mojca and Mueller, Reinhold D. and Nolan, Tania and O’Sullivan, Denise M. and Pfaffl, Michael W. and Rödiger, Stefan and Romsos, Erica L. and Shipley, Gregory L. and Taly, Valerie and Untergasser, Andreas and Wittwer, Carl T. and Bustin, Stephen A. and Vandesompele, Jo (2020) The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. Clinical Chemistry, 66 (8). pp. 1012-1029. ISSN 1530-8561

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Official URL: http://dx.doi.org/10.1093/clinchem/hvaa125

Abstract

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

Item Type: Journal Article
Faculty: Faculty of Health, Education, Medicine & Social Care
SWORD Depositor: Symplectic User
Depositing User: Symplectic User
Date Deposited: 13 Aug 2020 14:16
Last Modified: 11 Jun 2021 13:58
URI: https://arro.anglia.ac.uk/id/eprint/705741

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