RT-qPCR Testing of SARS-CoV-2: A Primer

Bustin, Stephen A. and Nolan, Tania (2020) RT-qPCR Testing of SARS-CoV-2: A Primer. International Journal of Molecular Sciences. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms21083004

Abstract

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

Item Type: Journal Article
Keywords: COVID-19, SARS, pandemic, reverse transcription, real-time fluorescence PCR
Faculty: Faculty of Health, Education, Medicine & Social Care
Depositing User: Ian Walker
Date Deposited: 24 Apr 2020 14:16
Last Modified: 24 Apr 2020 15:45
URI: http://arro.anglia.ac.uk/id/eprint/705443

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