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A multicentre validation of Metasin: a molecular assay for the intraoperative assessment of sentinel lymph nodes from breast cancer patients

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posted on 2023-07-26, 14:53 authored by Priya Sai-Giridhar, Salma Al-Ramadhani, Dilushana George, Preethi Gopinath, William Andrews, Samar Jader, Shelley Brown, Alison Findlay, Evdokia Arkoumani, Salam Al-Sam, Jolanta G McKenzie, Howard Bradpiece, Stephanie Jenkins, Anthony Aylwin, Simon Holt, Yousef Sharaiha, Constantinos Yiangou, Avi Agrawal, Amanda McDowell, Francis G Gabriel, Margaret Jeffrey, Neerja Agrawal, Ian A Cree, Robert E Mansel, Mo Keshtgar, Nuala McDermott, Soha El Sheikh, David Wellsted, Jade Collard, Hema Chaplin, Olfert Landt, Stephen A. Bustin, Maryse Sundaresan, Vasi Sundaresan
Aims: Treatment strategies for breast cancer continue to evolve. No uniformity exists in the UK for the management of node‐positive breast cancer patients. Most centres continue to use conventional histopathology of sampled sentinel lymph nodes (SLNs), which requires delayed axillary clearance in up to 25% of patients. Some use touch imprint cytology or frozen section for intraoperative testing, although both have inherent sensitivity issues. An intraoperative molecular diagnostic approach helps to overcome some of these limitations. The aim of this study was to assess the clinical effectiveness of Metasin, a molecular method for the intraoperative evaluation of SLNs. Methods and results: RNA from 3296 lymph nodes from 1836 patients undergoing SLN assessment was analysed with Metasin. Alternate slices of tissue were examined in parallel by histology. Cases deemed to be discordant were analysed by protein gel electrophoresis. There was concordance between Metasin and histology in 94.1% of cases, with a sensitivity of 92% [95% confidence interval (CI) 88–94%] and a specificity of 97% (95% CI 95–97%). Positive and negative predictive values were 88% and 98%, respectively. Over half of the discordant cases (4.4%) were ascribed to tissue allocation bias (TAB). Conclusions: Clinical validation of the Metasin assay suggests that it is sufficiently sensitive and specific to make it fit for purpose in the intraoperative setting.

History

Refereed

  • Yes

Volume

68

Issue number

6

Page range

875-887

Publication title

Histopathology

ISSN

1365-2559

Publisher

Wiley

File version

  • Published version

Language

  • eng

Legacy posted date

2020-02-25

Legacy creation date

2020-02-25

Legacy Faculty/School/Department

ARCHIVED Faculty of Medical Science (until September 2018)

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