Culture of Pulmonary Arterial Endothelial Cells from Pulmonary Artery Catheter Balloon Tips: Considerations for Use in Pulmonary Vascular Disease

Ventetuolo, Corey and Aliotta, Jason and Braza, Julie and Chichger, Havovi and Dooner, Mark and McGuirl, Donald and Mullin, Chris and Newton, Julie and Pereira, Mandy and Princiotto, Amy and Quesenberry, Peter and Walsh, Thomas and Whittenhall, Mary and Klinger, James R. and Harrington, Elizabeth O. (2020) Culture of Pulmonary Arterial Endothelial Cells from Pulmonary Artery Catheter Balloon Tips: Considerations for Use in Pulmonary Vascular Disease. European Respiratory Journal, 55. p. 1901313. ISSN 1399-3003

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Official URL: http://dx.doi.org/10.1183/13993003.01313-2019

Abstract

Endothelial dysfunction is a hallmark of pulmonary arterial hypertension (PAH) but there are no established methods to study pulmonary artery endothelial cells (PAECs) from living patients. We sought to culture PAECs from pulmonary artery catheter (PAC) balloons used during right heart catheterisation (RHC), to characterise successful culture attempts and to describe PAEC behavior. PAECs were grown in primary culture to confluence and endothelial cell phenotype confirmed. Standard assays for apoptosis, migration, and tube formation were performed between passage 3–8. We collected 49 PAC tips from 45 subjects with successful PAEC culture from 19 (39%) balloons. There were no differences in subject demographic or RHC procedural details in successful versus unsuccessful attempts. There was a higher but nonsignificant proportion of successful (10/19, 53%) versus unsuccessful (9/30, 30%) attempts from subjects who met hemodynamic criteria for PAH (p=0.10). Successful culture was more likely in subjects with lower cardiac index (p=0.03) and higher pulmonary vascular resistance (p=0.04). PAECs from a subject with idiopathic PAH were apoptosis resistant compared to commercial PAECs (p=0.04) and had reduced migration compared to PAECs from a subject with portopulmonary hypertension with high cardiac output (p=0.01). PAECs from a subject with HIV-associated PAH formed fewer (p=0.01) and shorter (p=0.02) vessel networks compared to commercial PAECs. Sustained culture and characterisation of PAECs from RHC balloons is feasible, especially in PAH with high hemodynamic burden. This technique may provide insight into endothelial dysfunction during PAH pathogenesis.

Item Type: Journal Article
Keywords: endothelial cells, cell culture techniques, pulmonary artery, apoptosis, hypertension, pulmonary
Faculty: Faculty of Science & Engineering
SWORD Depositor: Symplectic User
Depositing User: Symplectic User
Date Deposited: 11 Dec 2019 12:17
Last Modified: 09 Sep 2021 18:53
URI: https://arro.anglia.ac.uk/id/eprint/705034

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