Barnett_2019.pdf (2.61 MB)
Ethylene glycol coated nanoceria protects against oxidative stress in human lens epithelium
journal contribution
posted on 2023-07-26, 14:49 authored by Belal I. Hanafy, Gareth W. V. Cave, Yvonne A. Barnett, Barbara K. PierscionekChronic diseases are rising in incidence and prevalence because of increases in life expectancy in many parts of the world coupled with advances in medicine which manage disease progression, rather than curing and alleviating the causes. Cataract is one such chronic condition. Identifying a therapeutic intervention that is successful in reversing or preventing cataracts may have applications for other chronic diseases of protein misfolding, such as diabetes and Alzheimer's disease as these have similar causation factors, notably oxidative stress and/or glycation. Cerium oxide nanoparticles (nanoceria) which have antioxidant, radioprotective and enzyme-mimetic properties have the potential to lead to an effective non-surgical treatment. However, nanoceria stability in physiological media is poor thus hindering their effective use in biomedical applications. Here we report a highly efficient one-pot synthesis of nanoceria (2–5 nm) coated with ethylene glycol, that is colloidally stable in physiological media and exhibits multiwavelength photoluminescence. The formulation, up to concentrations of 200 μg ml−1, was not toxic to human lens epithelial cells and had no adverse effect on the cellular morphology or proliferation rate. More significantly, these nanoceria showed protective effects against oxidative stress induced by hydrogen peroxide in lens epithelial cells. Electron microscopy studies show the internalization and cytoplasmic localization of the nanoceria was found to be largely in the perinuclear region.
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Refereed
- Yes
Volume
9Issue number
29Page range
16596-16605Publication title
RSC AdvancesISSN
2046-2069External DOI
Publisher
Royal Society of ChemistryFile version
- Published version
Language
- eng
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Legacy posted date
2019-12-06Legacy creation date
2019-12-06Legacy Faculty/School/Department
Faculty of Science & EngineeringUsage metrics
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