Bustin, Stephen A. (2017) How to speed up the polymerase chain reaction. Biomolecular Detection and Quantification, 12. pp. 10-14. ISSN 2214-7535
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Official URL: https://doi.org/10.1016/j.bdq.2017.05.002
Abstract
Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
| Item Type: | Journal Article |
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| Keywords: | Real-time PCR, Taq polymerase, Denaturation, Annealing, Polymerisation |
| Faculty: | ARCHIVED Faculty of Medical Science (until September 2018) |
| Depositing User: | Lisa Blanshard |
| Date Deposited: | 23 Jan 2019 09:44 |
| Last Modified: | 09 Sep 2021 18:58 |
| URI: | https://arro.anglia.ac.uk/id/eprint/704047 |
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