Measurement of cortisol in saliva: a comparison of measurement error within and between international academic-research laboratories

Calvi, Jessica, Brann Benson, Victoria, Brindle, Eleanor, Bristow, Matt, De, Alpana, Entringer, Sonja Entringer, Findlay, Helen, Heim, Christine, Hodges, Eric A., Klawitter, Heiko, Lupien, Sonia, Rus, Holly M., Tiemensma, Jitske, Verlezza, Silvanna, Walker, Claire-Dominique and Granger, Douglas A. (2017) Measurement of cortisol in saliva: a comparison of measurement error within and between international academic-research laboratories. BMC Research Notes, 10. p. 479. ISSN 1756-0500

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Objective: Hundreds of scientific publications are produced annually that involve the measurement of cortisol in saliva. Intra- and inter-laboratory variation in salivary cortisol results has the potential to contribute to cross- study inconsistencies in findings, and the perception that salivary cortisol results are unreliable. This study rigor- ously estimates sources of measurement variability in the assay of salivary cortisol within and between established international academic-based laboratories that specialize in saliva analyses. One hundred young adults (Mean age: 23.10 years; 62 females) donated 2 mL of whole saliva by passive drool. Each sample was split into multiple- 100 µL aliquots and immediately frozen. One aliquot of each of the 100 participants’ saliva was transported to academic laboratories (N = 9) in the United States, Canada, UK, and Germany and assayed for cortisol by the same commercially available immunoassay. Results: 1.76% of the variance in salivary cortisol levels was attributable to differences between duplicate assays of the same sample within laboratories, 7.93% of the variance was associated with differences between laboratories, and 90.31% to differences between samples. In established-qualified laboratories, measurement error of salivary cortisol is minimal, and inter-laboratory differences in measurement are unlikely to have a major influence on the determined values.

Item Type: Journal Article
Keywords: Salivary cortisol, Inter-assay, Intra-assay, Inter-laboratory variation
Faculty: ARCHIVED Faculty of Science & Technology (until September 2018)
Depositing User: Dr Matt Bristow
Date Deposited: 04 Oct 2017 12:55
Last Modified: 10 May 2022 09:15

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