Proximity assays for sensitive quantification of proteins

Greenwood, Christina, Ruff, David, Kirvell, Sara, Johnson, Gemma L., Dhillon, Harvinder S. and Bustin, Stephen A. (2015) Proximity assays for sensitive quantification of proteins. Biomolecular Detection and Quantification, 4. pp. 10-16. ISSN 2214-7535

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Abstract

Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.

Item Type:	Journal Article
Keywords:	Immunoassays, Immuno-PCR, Proximity ligation assay, Proximity extension assay, In situ assays
Faculty:	ARCHIVED Faculty of Medical Science (until September 2018)
Depositing User:	Repository Admin
Date Deposited:	21 Sep 2015 13:29
Last Modified:	09 Sep 2021 19:01
URI:	https://arro.anglia.ac.uk/id/eprint/578556

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