The need for transparency and good practices in the qPCR literature

Bustin, Stephen A.; Benes, Vladimir; Garson, Jeremy A.; Hellemans, Jan; Huggett, Jim F.; Kubista, Mikael; Mueller, Reinhold D.; Nolan, Tania; Pfaffl, Michael W.; Shipley, Gregory; Wittwer, Carl T.; Schjerling, Peter; Day, Philip J.; Abreu, Mónica; Aguado, Begoña; Beaulieu, Jean-François; Beckers, Anneleen; Bogaert, Sara; Browne, John A.; Carrasco-Ramiro, Fernando; Ceelen, Liesbeth; Ciborowski, Kate; Cornillie, Pieter; Coulon, Stephanie; Cuypers, Ann; De Brouwer, Sara; De Ceuninck, Leentje; De Craene, Jurgen; De Naeyer, Hélène; De Spiegelaere, Ward; Deckers, Kato; Dheedene, Annelies; Durinck, Kaat; Ferreira-Teixeira, Margarida; Fieuw, Annelies; Gallup, Jack M.; Gonzalo-Flores, Sandra; Goossens, Karen; Heindryckx, Femke; Herring, Elizabeth; Hoenicka, Hans; Icardi, Laura; Jaggi, Rolf; Javad, Farzad; Karampelias, Michael; Kibenge, Frederick; Kibenge, Molly; Kumps, Candy; Lambertz, Irina; Lammens, Tim; Markey, Amelia; Messiaen, Peter; Mets, Evelien; Morais, Sofia; Mudarra-Rubio, Alberto; Nakiwala, Justine; Nelis, Hilde; Olsvik, Pal A.; Pérez-Novo, Claudina; Plusquin, Michelle; Remans, Tony; Rihani, Ali; Rodrigues-Santos, Paulo; Rondou, Pieter; Sanders, Rebecca; Schmidt-Bleek, Katharina; Skovgaard, Kerstin; Smeets, Karen; Tabera, Laura; Toegel, Stefan; Van Acker, Tim; Van den Broeck, Wim; Van der Meulen, Joni; Van Gele, Mireille; Van Peer, Gert; Van Poucke, Mario; Van Roy, Nadine; Vergult, Sarah; Wauman, Joris; Tshuikina-Wiklander, Marina; Willems, Erik; Zaccara, Sara; Zeka, Fjoralba; Vandesompele, Jo

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The need for transparency and good practices in the qPCR literature

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posted on 2023-07-26, 13:27 authored by Stephen A. Bustin, Vladimir Benes, Jeremy A. Garson, Jan Hellemans, Jim F. Huggett, Mikael Kubista, Reinhold D. Mueller, Tania Nolan, Michael W. Pfaffl, Gregory Shipley, Carl T. Wittwer, Peter Schjerling, Philip J. Day, Mónica Abreu, Begoña Aguado, Jean-François Beaulieu, Anneleen Beckers, Sara Bogaert, John A. Browne, Fernando Carrasco-Ramiro, Liesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Hélène De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M. Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michael Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A. Olsvik, Claudina Pérez-Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, Jo Vandesompele

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

History

Refereed

Yes

Volume

10

Issue number

11

Page range

1063-1067

Publication title

Nature Methods

ISSN

1548-7105

External DOI

https://doi.org/10.1038/nmeth.2697

Publisher

Springer

Language

other

Official URL

https://doi.org/10.1038/nmeth.2697

Legacy posted date

2013-11-05

Legacy Faculty/School/Department

ARCHIVED Faculty of Health, Social Care & Education (until September 2018)

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CC BY 4.0

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File(s) not publicly available

The need for transparency and good practices in the qPCR literature

History

Refereed

Volume

Issue number

Page range

Publication title

ISSN

External DOI

Publisher

Language

Official URL

Legacy posted date

Legacy Faculty/School/Department

Usage metrics

Categories

Keywords

Licence

Exports