Anglia Ruskin Research Online (ARRO)
Browse

File(s) not publicly available

Analysis of mRNA expression by real-time PCR

chapter
posted on 2023-07-26, 13:13 authored by Stephen A. Bustin, Tania Nolan
The last few years have witnessed the transformation of the real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR) from an experimental technology into a mainstream scientific tool for the detection and quantification of RNA with an enormous range of uses in basic research, molecular medicine and biotechnology. The continuous improvement of reagents and instruments, combined with the trend towards high throughput and miniaturisation, is likely to reinforce that pre-eminence and continue to open up new application areas. Nonetheless, although in principle undoubtedly a straightforward technology, the reliability of RT-qPCR assays depends a series of sequential steps that include careful experimental design, optimisation and validation, which must be implemented pragmatically to obtain meaningful, biologically relevant data.

History

Refereed

  • Yes

Number of pages

284

Publisher

Caister Academic Press

Place of publication

Poole, UK

Title of book

Real-Time PCR: Advanced Technologies and Applications

ISBN

978-1-908230-22-5

Editors

Nick A. Saunders, Martin A. Lee

Language

  • other

Legacy posted date

2013-06-24

Legacy Faculty/School/Department

ARCHIVED Faculty of Health, Social Care & Education (until September 2018)

Usage metrics

    ARU Outputs

    Categories

    No categories selected

    Keywords

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC