Real-time quantitative PCR, pathogen detection and MIQE

Johnson, Gemma L., Nolan, Tania and Bustin, Stephen A. (2013) Real-time quantitative PCR, pathogen detection and MIQE. In: PCR Detection of Microbial Pathogens: Second Edition. Methods in Molecular Biology, 943 . Humana Press, New York, NY, pp. 1-16. ISBN 9781603273534

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Abstract

Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the "Minimal Information for the Publication of real-time PCR" (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.

Item Type:	Book Chapter
Faculty:	ARCHIVED Faculty of Health, Social Care & Education (until September 2018)
Depositing User:	Repository Admin
Date Deposited:	22 Apr 2013 10:39
Last Modified:	31 Mar 2022 15:11
URI:	https://arro.anglia.ac.uk/id/eprint/283412

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