How to speed up the polymerase chain reaction

Bustin, Stephen A. (2017) How to speed up the polymerase chain reaction. Biomolecular Detection and Quantification, 12. pp. 10-14. ISSN 2214-7535

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Official URL: https://doi.org/10.1016/j.bdq.2017.05.002

Abstract

Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

Item Type: Journal Article
Keywords: Real-time PCR, Taq polymerase, Denaturation, Annealing, Polymerisation
Faculty: ARCHIVED Faculty of Medical Science (until September 2018)
Depositing User: Lisa Blanshard
Date Deposited: 23 Jan 2019 09:44
Last Modified: 14 Nov 2019 16:10
URI: http://arro.anglia.ac.uk/id/eprint/704047

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