Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

Prattes, Juergen and Hoenigl, Martin and Zinke, Stefanie E.-M. and Heldt, Sven and Eigl, Susanne and Johnson, Gemma L. and Bustin, Stephen and Stelzl, Evelyn and Kessler, Harald H. (2018) Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study. Mycoses, 61 (6). pp. 355-359. ISSN 1439-0507

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Official URL: http://dx.doi.org/10.1111/myc.12757

Abstract

The newly developed AspID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis (IPA) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the AspID assay were 94.1% (95% CI 73.3-99.9), 76.5% (95% CI 50.1-93.2), 4 (95% CI 1.7-9.5) and 0.1 (95% CI 0.01-0.5) respectively.

Item Type: Journal Article
Keywords: Science & Technology, Life Sciences & Biomedicine, Dermatology, Mycology, Asp, ID, BALF, PCR, Invasive Pulmonary Aapergillosis, Bronchoalveolar lavage fluid, Lateral-flow device, Hematological malignancies, Galactomannan levels, Antifungal therapy, Fungal diseases, Diagnosis, Samples
Faculty: Faculty of Medical Science
SWORD Depositor: Symplectic User
Depositing User: Symplectic User
Date Deposited: 31 Aug 2018 11:42
Last Modified: 31 Aug 2018 11:46
URI: http://arro.anglia.ac.uk/id/eprint/703533

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