Ethanol-Based Proliposome Delivery Systems of Paclitaxel for In Vitro Application Against Brain Cancer Cells

Najlah, Mohammad and Jain, Mohit and Wan, Ka-Wai and Ahmed, Waqar and Albed Alhnan, Mohamed and Phoenix, David A. and Taylor, Kevin M. G. and Elhissi, Abdelbary M. A. (2018) Ethanol-Based Proliposome Delivery Systems of Paclitaxel for In Vitro Application Against Brain Cancer Cells. Journal of Liposome Research, 28 (1). pp. 74-85. ISSN 1532-2394

[img] Text
Accepted Version
Available under the following license: Creative Commons Attribution Non-commercial No Derivatives.

Download (762kB)
[img] Text
Supplemental Material
Available under the following license: Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB)
Official URL: http://dx.doi.org/10.1080/08982104.2016.1259628

Abstract

In this study the anticancer activity of paclitaxel-loaded nano-liposomes on glioma cell lines was investigated. Soya phosphatidylcholine: cholesterol (SPC:Chol), hydrogenated soya phosphatidylcholine: cholesterol (HSPC:Chol) or dipalmitoylphosphatidylcholine: cholesterol (DPPC:Chol) in 1:1 mole ratio were used to prepare ethanol-based proliposomes. Following hydration of proliposomes, the size of resulting vesicles was subsequently reduced to nanometre scale via probe-sonication. The resulting formulations were characterised in terms of size, zeta potential and morphology of the vesicles, and entrapment efficiency of paclitaxel (PX) as well as the final pH of the preparations. DPPC-liposomes entrapped 35-92% of PX compared to 27-74% and 25-60% entrapped by liposomes made from SPC and HSPC formulations respectively, depending on drug concentration. The entrapment efficiency of liposomes was dependent on the lipid bilayer properties and ability of PX to modify surface charge of the vesicles. In vitro cytotoxicity studies revealed that PX-liposome formulations were more selective at inhibiting the malignant cells. The cytotoxicity of PX-liposomes was dependent on their drug entrapment efficiency. This study has shown PX-liposomes generated from proliposomes have selective activity against glioma cell lines, and the synthetic DPPC phospholipid was most suitable for maximised drug entrapment and highest activity against the malignant cells in vitro.

Item Type: Journal Article
Keywords: proliposome, cytotoxicity, paclitaxel, entrapment efficiency, phospholipids, cell culture
Faculty: Faculty of Medical Science
Depositing User: Dr Mohammad Najlah
Date Deposited: 14 Nov 2016 11:18
Last Modified: 26 Oct 2018 15:51
URI: http://arro.anglia.ac.uk/id/eprint/701120

Actions (login required)

Edit Item Edit Item