Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease

Elliott, Timothy R. and Rayment, Neil B. and Hudspith, Barry N. and Hands, Rebecca E. and Taylor, Kirstin and Parkes, Gareth C. and Prescott, Natalie J. and Petrovska, Liljana and Hermon-Taylor, John and Brostoff, Jonathan and Boussioutas, Alex and Mathew, Christopher G. and Bustin, Stephen A. and Sanderson, Jeremy D. (2015) Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease. BMC Gastroenterology, 15. p. 75. ISSN 1471-230X

[img]
Preview
Text
Published Version
Available under the following license: Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Preview
Official URL: http://dx.doi.org/10.1186/s12876-015-0305-3

Abstract

Background: Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods: Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results: E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion: In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation.

Item Type: Journal Article
Keywords: Crohn’s disease, Escherichia coli, Macrophages
Faculty: ARCHIVED Faculty of Medical Science (until September 2018)
Depositing User: Repository Admin
Date Deposited: 30 Sep 2015 15:11
Last Modified: 04 Dec 2019 10:35
URI: http://arro.anglia.ac.uk/id/eprint/578939

Actions (login required)

Edit Item Edit Item