The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Huggett, Jim F. and Foy, Carole A. and Benes, Vladimir and Emslie, Kerry and Garson, Jeremy A. and Haynes, Ross and Hellemans, Jan and Kubista, Mikael and Mueller, Reinhold D. and Nolan, Tania and Pfaffl, Michael W. and Shipley, Gregory L. and Vandesompele, Jo and Wittwer, Carl T. and Bustin, Stephen A. (2013) The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chemistry, 59 (6). pp. 892-902. ISSN 1530-8561

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Official URL: http://dx.doi.org/10.1373/clinchem.2013.206375

Abstract

There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation. Adoption of these guidelines by the scientific community will help to standardize experimental protocols, maximize efficient utilization of resources, and enhance the impact of this promising new technology. © 2013 American Association for Clinical Chemistry

Item Type: Journal Article
Faculty: ARCHIVED Faculty of Health, Social Care & Education (until September 2018)
Depositing User: Repository Admin
Date Deposited: 22 Apr 2013 10:51
Last Modified: 14 Nov 2019 16:03
URI: http://arro.anglia.ac.uk/id/eprint/283432

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