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Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS)

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journal contribution
posted on 2023-07-26, 14:51 authored by Marie W. Pettit, Paul D. R. Dyer, John C. Mitchell, Peter C. Griffiths, Bruce D. Alexander, Beatrice N. Cattoz, Richard K. Heenan, Stephen M. King, Ralf Schweins, Frank Pullen, Stephen R. Wicks, Simon C. W. Richardson
An increasing human population requires a secure food supply and a cost effective, oral vaccine delivery system for livestock would help facilitate this end. Recombinant antigen adsorbed onto silica beads and coated with myristic acid, was released (∼15% (w/v)) over 24 h at pH 8.8. At pH 2, the myristic acid acted as an enteric coating, protecting the antigen from a variety of proteases. The antigen adsorbed onto silica particles, coated in myristic acid had a conserved secondary structure (measured by circular dichroism (CD) spectroscopy) following its pH-triggered release. Small angle neutron scattering (SANS) was used to measure the thickness of the adsorbed antigen, finding that its adsorbed conformation was slightly greater than its solution radius of gyration, i.e. 120-160 Å. The addition of myristic acid led to a further increase in particle size, with scattering data consistent with an acid thickness slightly greater than a monolayer of fully extended alkyl chains and a degree of hydration of around 50%. Whilst adsorbed onto the silica and coated in myristic acid, the protein was stable over 14 days at 42 °C, indicating a reduced need for cold chain storage. These data indicate that further investigation is warranted into the development of this technology.

History

Refereed

  • Yes

Volume

468

Issue number

1-2

Page range

264-271

Publication title

International Journal of Pharmaceutics

ISSN

1873-3476

Publisher

Elsevier

File version

  • Published version

Language

  • eng

Legacy posted date

2020-01-15

Legacy creation date

2020-01-15

Legacy Faculty/School/Department

ARCHIVED Faculty of Science & Technology (until September 2018)

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