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How to speed up the polymerase chain reaction

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journal contribution
posted on 2023-07-26, 14:32 authored by Stephen A. Bustin
Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

History

Refereed

  • Yes

Volume

12

Page range

10-14

Publication title

Biomolecular Detection and Quantification

ISSN

2214-7535

Publisher

Elsevier

File version

  • Published version

Language

  • eng

Legacy posted date

2019-01-23

Legacy creation date

2019-01-23

Legacy Faculty/School/Department

ARCHIVED Faculty of Medical Science (until September 2018)

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