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Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

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journal contribution
posted on 2023-07-26, 14:25 authored by Juergen Prattes, Martin Hoenigl, Stefanie E.-M. Zinke, Sven Heldt, Susanne Eigl, Gemma L. Johnson, Stephen A. Bustin, Evelyn Stelzl, Harald H. Kessler
The newly developed AspID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis (IPA) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the AspID assay were 94.1% (95% CI 73.3-99.9), 76.5% (95% CI 50.1-93.2), 4 (95% CI 1.7-9.5) and 0.1 (95% CI 0.01-0.5) respectively.

History

Refereed

  • Yes

Volume

61

Issue number

6

Page range

355-359

Publication title

Mycoses

ISSN

1439-0507

Publisher

Wiley

File version

  • Published version

Language

  • eng

Legacy posted date

2018-08-31

Legacy creation date

2018-08-31

Legacy Faculty/School/Department

ARCHIVED Faculty of Medical Science (until September 2018)