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Proximity assays for sensitive quantification of proteins

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posted on 2023-07-26, 13:39 authored by Christina Greenwood, David Ruff, Sara Kirvell, Gemma L. Johnson, Harvinder S. Dhillon, Stephen A. Bustin
Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.

History

Refereed

  • Yes

Volume

4

Page range

10-16

Publication title

Biomolecular Detection and Quantification

ISSN

2214-7535

Publisher

Elsevier

File version

  • Published version

Language

  • eng

Legacy posted date

2015-09-21

Legacy creation date

2019-05-16

Legacy Faculty/School/Department

ARCHIVED Faculty of Medical Science (until September 2018)

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