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Real-time quantitative PCR, pathogen detection and MIQE
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posted on 2023-07-26, 12:58 authored by Gemma L. Johnson, Tania Nolan, Stephen A. BustinNucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the "Minimal Information for the Publication of real-time PCR" (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.
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Refereed
- Yes
Volume
943Page range
1-16Series
Methods in Molecular BiologyExternal DOI
Publisher
Humana PressPlace of publication
New York, NYTitle of book
PCR Detection of Microbial Pathogens: Second EditionISBN
9781603273534Language
- other
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Legacy posted date
2013-04-22Legacy Faculty/School/Department
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