Application of a urine and hair validated LC-MS/MS method to determine the effect of hair colour on the incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into hair in the rat.

Nisbet, Lorna and Venson, Rafael and Wylie, Fiona and Scott, Karen S. (2017) Application of a urine and hair validated LC-MS/MS method to determine the effect of hair colour on the incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into hair in the rat. Journal of Analytical Toxicology. ISSN 1945-2403

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Official URL: https://doi.org/10.1093/jat/bkx053

Abstract

NBOMes are a group of new psychoactive substances derived from phenethylamines. Recreational abuse is thought to have begun in 2010 and they are commonly associated with the “club drug” scene. They are administered in liquid form or as blotters due to their high potency. An LC-MS/MS method was validated using SWGTOX parameters for the detection of 25B-, 25C- and 25I-NBOMe using 25B-NBOMe-D3 as internal standard for urine and hair. Calibration graphs with R2 values >0.99 were observed for urine and hair for concentrations ranging from 0.1 -100 ng/mL and 0.025-2.5 ng/mg respectively. Urine LODs ranged from 5-25 pg/mL and had an LOQ of 50 pg/mL. Hair LOD and LOQs ranged from 3-5 pg/mg and 6.25-12.5 pg/mg respectively. Intra and inter-day precision was <20% and accuracy was within ± 20% for both matrices. The method was shown to be selective for both exogenous and endogenous compounds. No matrix effects were observed for either matrix. LLE recovery ranged from 90-103% for urine samples and SPE recovery ranged from 80-107% for hair samples. Long-Evans rats (n=55) were administered 25B-, 25C- or 25I-NBOMe at doses ranging from 30-300 µg/kg over a period of 10 days. Rats were shaved prior to their first dose and re-shaved after the 10-day period. Hair was separated by colour (black: n=55 and white: n=55) and analysed using the validated LC-MS/MS method to assess the impact hair colour has on the incorporation of these drugs. All drugs were successfully detected in black hair. 25B-NBOMe from rats receiving the highest dose and 25C-NBOMe from rats receiving the medium and high doses were quantified in white hair. 25I-NBOMe was detected but fell below the limit of quantification. A dose-dependent concentration increase was observed in the black hair. All pooled urine samples tested positive for their expected NBOMes.

Item Type: Journal Article
Additional Information: The version of record is available online at: https://doi.org/10.1093/jat/bkx053
Keywords: NBOMe, LC-MS/MS, hair testing, urinalysis
Faculty: Faculty of Science & Technology
Depositing User: Unnamed user with email Lorna.Nisbet@anglia.ac.uk
Date Deposited: 04 Jul 2017 08:05
Last Modified: 26 Jul 2017 10:31
URI: http://arro.anglia.ac.uk/id/eprint/701903

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