Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh.

Ansari, Wahedul Karim and Parvej, Md Shafiullah and El Zowalaty, Mohamed E. and Jackson, Sally and Bustin, Stephen A. and Ibrahim, Adel K and El Zowalaty, Ahmed E. and Rahman, Md Tanvir and Zhang, Han and Khan, Mohammad Ferdousur Rahman and Ahamed, Md Mostakin and Rahman, Md Fasiur and Rahman, Marzia and Nazir, K H M Nazmul Hussain and Ahmed, Sultan and Hossen, Md Liakot and Kafi, Md Abdul and Yamage, Mat and Debnath, Nitish C. and Ahmed, Graba and Ashour, Hossam M. and Masudur Rahman, Md and Noreddin, Ayman and Rahman, Md Bahanur (2016) Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh. Veterinary microbiology, 193. pp. 49-59. ISSN 1873-2542

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Abstract

Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2191 oropharyngeal swabs as well as 1725 sera samples were collected from live birds including duck and chicken in different locations in Bangladesh between the years of 2013 and 2014. Samples were tested using virus isolation, serological tests and molecular methods of RT-PCR. Influenza A viruses were detected using reverse transcription PCR targeting the virus matrix (M) gene in 41/4360 (0.94%) samples including both cloacal and oropharyngeal swab samples, 31 of which were subtyped as H5N1 using subtype-specific primers. Twenty-one live H5N1 virus isolates were recovered from those 31 samples. Screening of 1,868 blood samples collected from the same birds using H5-specific ELISA identified 545/1603 (34%) positive samples. Disconcertingly, an analysis of 221 serum samples collected from vaccinated layer chicken in four districts revealed that only 18 samples (8.1%) were seropositive for anti H5 antibodies, compared to unvaccinated birds (n=105), where 8 samples (7.6%) were seropositive. Our result indicates that the vaccination program as currently implemented should be reviewed and updated. In addition, surveillance programs are crucial for monitoring the efficacy of the current poultry vaccinations programs, and to monitor the circulating AIV strains and emergence of AIV subtypes in Bangladesh.

Item Type: Journal Article
Keywords: Avian Influenza, Reverse transcription polymerase chain reaction, H5N1
Depositing User: Unnamed user with email stephen.bustin@anglia.ac.uk
Date Deposited: 07 Dec 2016 12:52
Last Modified: 29 Jun 2017 12:07
URI: http://arro.anglia.ac.uk/id/eprint/700900

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