A collaborative exercise on DNA methylation based body fluid typing

Jung, Sang-Eun and Cho, Sohee and Antunes, Joana and Gomes, Iva and Uchimoto, Mari L. and Oh, Yu Na and Di Giacomo, Lisa and Schneider, Peter M. and Park, Min Sun and van der Meer, Dieudonne and Williams, Graham and McCord, Bruce and Ahn, Hee-Jung and Choi, Dong Ho and Lee, Yang Han and Lee, Soong Deok and Lee, Hwan Young (2016) A collaborative exercise on DNA methylation based body fluid typing. Electrophoresis. ISSN 01730835 (Accepted)

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Official URL: http://doi.org/10.1002/elps.201600256

Abstract

A collaborative exercise on DNA methylation based body fluid identification was conducted by seven laboratories. For this project, a multiplex methylation SNaPshot reaction composed of seven CpG markers was used for the identification of four body fluids, including blood, saliva, semen, and vaginal fluid. A total of 30 specimens were prepared and distributed to participating laboratories after thorough testing. The required experiments included four increasingly complex tasks: (1) CE of a purified single-base extension reaction product, (2) multiplex PCR and multiplex single-base extension reaction of bisulfite-modified DNA, (3) bisulfite conversion of genomic DNA, and (4) extraction of genomic DNA from body fluid samples. In tasks 2, 3 and 4, one or more mixtures were analyzed, and specimens containing both known and unknown body fluid sources were used. Six of the laboratories generated consistent body fluid typing results for specimens of bisulfite-converted DNA and genomic DNA. One laboratory failed to set up appropriate conditions for capillary analysis of reference single-base extension products. In general, variation in the values obtained for DNA methylation analysis between laboratories increased with the complexity of the required experiments. However, all laboratories concurred on the interpretation of the DNA methylation profiles produced. Although the establishment of interpretational guidelines on DNA methylation based body fluid identification has yet to be performed, this study supports the addition of DNA methylation profiling to forensic body fluid typing.

Item Type: Journal Article
Additional Information: This is the peer reviewed version of the following article - Jung, S.-E., Cho, S., Antunes, J., Gomes, I., Uchimoto, M. L., Oh, Y. N., Di Giacomo, L., Schneider, P. M., Park, M. S., van der Meer, D., Williams, G., McCord, B., Ahn, H.-J., Choi, D. H., Lee, Y. H., Lee, S. D. and Lee, H. Y. (2016), A collaborative exercise on DNA methylation based body fluid typing. Electrophoresis - which has been published in final form at [http://doi.org/10.1002/elps.201600256]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Keywords: Body fluid identification, Collaborative exercise, DNA methylation
Faculty: Faculty of Science & Technology
Depositing User: Ian Walker
Date Deposited: 03 Oct 2016 08:29
Last Modified: 19 Sep 2017 01:02
URI: http://arro.anglia.ac.uk/id/eprint/700896

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