Identification of platelet function defects by multi-parameter assessment of thrombus formation.

de Witt, Susanne M. and Swieringa, Frauke and Cavill, Rachel and Lamers, Moniek M. E. and van Kruchten, Roger and Mastenbroek, Tom and Baaten, Constance and Coort, Susan and Pugh, Nicholas and Schulz, Ansgar and Scharrer, Inge and Jurk, Kerstin and Zieger, Barbara and Clemetson, Kenneth J. and Farndale, Richard W. and Heemskerk, Johan W. M. and Cosemans, Judith M. E. M. (2014) Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nature Communications. ISSN 2041-1723

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Abstract

Assays measuring platelet aggregation (thrombus formation) at arterial shear rate mostly use collagen as only platelet-adhesive surface. Here we report a multi-surface and multi-parameter flow assay to characterize thrombus formation in whole blood from healthy subjects and patients with platelet function deficiencies. A systematic comparison is made of 52 adhesive surfaces with components activating the main platelet-adhesive receptors, and of eight output parameters reflecting distinct stages of thrombus formation. Three types of thrombus formation can be identified with a predicted hierarchy of the following receptors: glycoprotein (GP)VI, C-type lectin-like receptor-2 (CLEC-2)>GPIb>α6β1, αIIbβ3>α2β1>CD36, α5β1, αvβ3. Application with patient blood reveals distinct abnormalities in thrombus formation in patients with severe combined immune deficiency, Glanzmann's thrombasthenia, Hermansky-Pudlak syndrome, May-Hegglin anomaly or grey platelet syndrome. We suggest this test may be useful for the diagnosis of patients with suspected bleeding disorders or a pro-thrombotic tendency.

Item Type: Journal Article
Additional Information: Citation: de Witt, S. M., Swieringa, F., Cavill, R., Lamers, M.E.M., Kruchten, R., Mastenbroek, T. Baaten, C., Coort, S., Pugh, N. Schulz, A., Scharrer, I., Jurk, K., Zieger, B. Clemetson, K.J., Farndale, R. W., Heemskerk, J. W. M. and Cosemans, J. M.E.M., 2014. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nature Communications, 5: 4257..
Faculty: Faculty of Science & Technology
Depositing User: Mr I Walker
Date Deposited: 07 Dec 2015 15:28
Last Modified: 04 Nov 2016 14:22
URI: http://arro.anglia.ac.uk/id/eprint/583322

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