Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals

Alsultan, Abdulrahman A. and Aboulmagd, Elsayed and Evans, Benjamin A. and Amyes, Sebastian G.B. (2014) Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals. Journal of Medical Microbiology. ISSN 0022-2615

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Abstract

Carbapenem-resistant Acinetobacter baumannii (CR-AB) represents a major health-care problem causing high rates of morbidity and mortality. This study investigated the clonality of CR-AB isolated from diabetic patients from different regions in Saudi Arabia as well as the relatedness of the β lactamases genes. A total of 64 non-repetitive CR-AB clinical isolates were collected from 16 different regions in Saudi Arabia from intensive care patients. Isolates were identified phenotypically by Vitek 2 compact system and genotypically by amplification of blaOXA-51-like gene. The target sequences were amplified by PCR and the clonal diversity of the isolates was explored by PFGE. Resistance studies revealed that the prevalence of imipenem and meropenem resistance was 92% and 96%, respectively, while the vast majority of the isolates were susceptible to tigecycline and colistin. In addition, blaVIM and blaOXA-23 were the most prevalent genes in the isolates under investigation while ISAba1 was the most dominant insertion sequence. PFGE results showed 13 clusters; clone H was dominant comprising 20 isolates from four hospitals followed by clones C and F comprising 11 isolates each from 3 and 6 hospitals, respectively. Moreover, the current study signified the clonal diversity of CR-AB in Saudi Arabia and showed the ability of some clones to infect patients in many different cities.

Item Type: Journal Article
Additional Information: Citation: Alsultan, A.A., Aboulmagd, E., Evans, B.A. and Amyes, S.G.B. 2014. Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals. Journal of Medical Microbiology, 63(11), pp.1460-1466 (online ahead of print: 8th August 2014).
Faculty: Faculty of Science & Technology
Depositing User: Mr I Walker
Date Deposited: 08 Dec 2014 12:14
Last Modified: 30 Jun 2017 09:37
URI: http://arro.anglia.ac.uk/id/eprint/336900

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