The need for transparency and good practices in the qPCR literature

Bustin, Stephen A. and Benes, Vladimir and Garson, Jeremy A. and Hellemans, Jan and Huggett, Jim F. and Kubista, Mikael and Mueller, Reinhold D. and Nolan, Tania and Pfaffl, Michael W. and Shipley, Gregory and Wittwer, Carl T. and Schjerling, Peter and Day, Philip J. and Abreu, Mónica and Aguado, Begoña and Beaulieu, Jean-François and Beckers, Anneleen and Bogaert, Sara and Browne, John A. and Carrasco-Ramiro, Fernando and Ceelen, Liesbeth and Ciborowski, Kate and Cornillie, Pieter and Coulon, Stephanie and Cuypers, Ann and De Brouwer, Sara and De Ceuninck, Leentje and De Craene, Jurgen and De Naeyer, Hélène and De Spiegelaere, Ward and Deckers, Kato and Dheedene, Annelies and Durinck, Kaat and Ferreira-Teixeira, Margarida and Fieuw, Annelies and Gallup, Jack M. and Gonzalo-Flores, Sandra and Goossens, Karen and Heindryckx, Femke and Herring, Elizabeth and Hoenicka, Hans and Icardi, Laura and Jaggi, Rolf and Javad, Farzad and Karampelias, Michael and Kibenge, Frederick and Kibenge, Molly and Kumps, Candy and Lambertz, Irina and Lammens, Tim and Markey, Amelia and Messiaen, Peter and Mets, Evelien and Morais, Sofia and Mudarra-Rubio, Alberto and Nakiwala, Justine and Nelis, Hilde and Olsvik, Pal A. and Pérez-Novo, Claudina and Plusquin, Michelle and Remans, Tony and Rihani, Ali and Rodrigues-Santos, Paulo and Rondou, Pieter and Sanders, Rebecca and Schmidt-Bleek, Katharina and Skovgaard, Kerstin and Smeets, Karen and Tabera, Laura and Toegel, Stefan and Van Acker, Tim and Van den Broeck, Wim and Van der Meulen, Joni and Van Gele, Mireille and Van Peer, Gert and Van Poucke, Mario and Van Roy, Nadine and Vergult, Sarah and Wauman, Joris and Tshuikina-Wiklander, Marina and Willems, Erik and Zaccara, Sara and Zeka, Fjoralba and Vandesompele, Jo (2013) The need for transparency and good practices in the qPCR literature. Nature Methods. ISSN 1548-7105 (Draft)

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Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Item Type: Journal Article
Additional Information: Citation: Bustin, S., et al., 2013. The need for transparency and good practices in the qPCR literature. Nature Methods, 10(11), pp.1063-1067..
Faculty: Faculty of Health, Social Care & Education (for research post September 2011)
Depositing User: Mr I Walker
Date Deposited: 05 Nov 2013 11:56
Last Modified: 03 Jul 2017 08:26
URI: http://arro.anglia.ac.uk/id/eprint/304968

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